Jump to main content
Jump to site search

Issue 9, 2015
Previous Article Next Article

STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments

Author affiliations

Abstract

The methodology termed scanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) has been used in this work to study the uptake of citrate stabilized gold nanoparticles (AuNPs) (average particle sizes of 23.5 ± 4.0 nm) into tissue samples upon in vitro exposure of the dissected gills of the Ruditapes philippinarum marine bivalve to the nanoparticle suspensions. The STEM-in-SEM methodology has been optimized for achieving optimum resolution under SEM low voltage operating conditions (20–30 kV). Based on scanning microscope assessments and resolution testing (SMART), resolutions well below 10 nm were appropriately achieved by working at magnifications over 100k×, with experimental sample thickness between 300 and 200 nm. These relatively thick slices appear to be stable under the beam and help avoid NP displacement during cutting. We herein show that both localizing of the internalized nanoparticles and imaging of ultrastructural disturbances in gill tissues are strongly accessible due to the improved resolution, even at sample thicknesses higher than those normally employed in standard TEM techniques at higher voltages. Ultrastructural imaging of bio-nano features in bioaccumulation experiments have been demonstrated in this study.

Graphical abstract: STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Sep 2014, accepted on 03 Feb 2015 and first published on 05 Feb 2015


Article type: Paper
DOI: 10.1039/C4AN01643B
Author version
available:
Download author version (PDF)
Citation: Analyst, 2015,140, 3082-3089
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments

    C. A. García-Negrete, M. C. Jiménez de Haro, J. Blasco, M. Soto and A. Fernández, Analyst, 2015, 140, 3082
    DOI: 10.1039/C4AN01643B

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements