Jump to main content
Jump to site search

Issue 21, 2014
Previous Article Next Article

An electrochemiluminescence biosensor for sensitive and selective detection of Hg2+ based on π–π interaction between nucleotides and ferrocene–graphene nanosheets

Author affiliations

Abstract

A solid-state electrochemiluminescence (ECL) biosensor based on a DNA-modified electrode platform that depends on the variation of π–π interaction before and after the binding of target analytes is put forward. The single-stranded DNA (ssDNA) probe was successfully assembled on the surface of a glassy carbon electrode (GCE), which was pre-modified with Ru(bpy)32+ complex and gold nanoparticles (GNPs). The ssDNA probe could strongly adsorb graphene due to the strong π–π interaction between nucleotides and graphene (GN), while in the presence of Hg2+, the conformational transformation of DNA from a single-stranded to a double-stranded structure resulted in inhibited adsorption of GN. With thymine (T)-rich ssDNA as a Hg2+ probe, we prepared the ECL biosensor by using ferrocene–graphene (Fc–GN) as a quenching unit to quench the ECL emission of Ru(bpy)32+, and the Hg2+ can be detected by quenching efficiency transformation when the Fc–GN gets away from Ru(bpy)32+. The biosensor exhibited a sensitive response to various ranges of concentration of Hg2+ with a detection limit of 18 pM. The ECL biosensor held great promise in the highly sensitive and selective detection of Hg2+ in natural water.

Graphical abstract: An electrochemiluminescence biosensor for sensitive and selective detection of Hg2+ based on π–π interaction between nucleotides and ferrocene–graphene nanosheets

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 20 Jan 2014, accepted on 29 Mar 2014 and first published on 31 Mar 2014


Article type: Paper
DOI: 10.1039/C4TB00115J
Citation: J. Mater. Chem. B, 2014,2, 3263-3270
  •   Request permissions

    An electrochemiluminescence biosensor for sensitive and selective detection of Hg2+ based on π–π interaction between nucleotides and ferrocene–graphene nanosheets

    B. Zhuo, Y. Li, A. Zhang, F. Lu, Y. Chen and W. Gao, J. Mater. Chem. B, 2014, 2, 3263
    DOI: 10.1039/C4TB00115J

Search articles by author