Jump to main content
Jump to site search

Issue 22, 2014
Previous Article Next Article

Effects of 3D nanocomposite bioceramic scaffolds on the immune response

Author affiliations


The interaction of new nanocomposite mesoporous glass/hydroxyapatite (MGHA) scaffolds with immune cells involved in both innate and acquired immunity has been studied in vitro as an essential aspect of their biocompatibility assessment. Since the immune response can be affected by the degradation products of bioresorbable scaffolds and scaffold surface changes, both processes have been evaluated. No alterations in proliferation and viability of RAW-264.7 macrophage-like cells were detected after culture on MGHA scaffolds which did not induce cell apoptosis. However, a slight cell size decrease and an intracellular calcium content increase were observed after contact of this cell line with MGHA scaffolds or their extracts. Although no changes in the percentages of RAW cells with low and high contents of reactive oxygen species (ROS) are observed by the treatment with 7 day extracts, this study has revealed modifications of these percentages after direct contact with scaffolds and by the treatment with 24 h extracts, related to the high reactivity/bioactivity of this MGHA nanocomposite at initial times. Furthermore, when normal fresh murine spleen cells were used as an experimental model closer to physiological conditions, no significant alterations in the activation of different immune cell subpopulations were detected in the presence of 24 h MGHA extract. MGHA scaffolds did not affect either the spontaneous apoptosis or intracellular cytokine expression (IL-2, IL-10, IFN-γ, and TNF-α) after 24 h treatment. The results obtained in the present study with murine immune cell subpopulations (macrophages, lymphocytes B, lymphocytes T and natural killer cells) support the biocompatibility of the MGHA material and suggest an adequate host tissue response to their scaffolds upon their implantation.

Graphical abstract: Effects of 3D nanocomposite bioceramic scaffolds on the immune response

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 17 Jan 2014, accepted on 28 Mar 2014 and first published on 31 Mar 2014

Article type: Paper
DOI: 10.1039/C4TB00106K
Citation: J. Mater. Chem. B, 2014,2, 3469-3479
  •   Request permissions

    Effects of 3D nanocomposite bioceramic scaffolds on the immune response

    M. Cicuéndez, P. Portolés, M. Montes-Casado, I. Izquierdo-Barba, M. Vallet-Regí and M. T. Portolés, J. Mater. Chem. B, 2014, 2, 3469
    DOI: 10.1039/C4TB00106K

Search articles by author