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Issue 20, 2014
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Backside-surface imprinting as a new strategy to generate specific plastic antibody materials

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Abstract

A backside protein-surface imprinting process is presented herein as a novel way to generate specific synthetic antibody materials. The template is covalently bonded to a carboxylated-PVC supporting film previously cast on gold, let to interact with charged monomers and surrounded next by another thick polymer. This polymer is then covalently attached to a transducing element and the backside of this structure (supporting film plus template) is removed as a regular “tape”. The new sensing layer is exposed after the full template removal, showing a high density of re-binding positions, as evidenced by SEM. To ensure that the templates have been efficiently removed, this re-binding layer was cleaned further with a proteolytic enzyme and solution washout. The final material was named MAPS, as in the back-side reading of SPAM, because it acts as a back-side imprinting of this recent approach. It was able to generate, for the first time, a specific response to a complex biomolecule from a synthetic material. Non-imprinted materials (NIMs) were also produced as blank and were used as a control of the imprinting process. All chemical modifications were followed by electrochemical techniques. This was done on a supporting film and transducing element of both MAPS and NIM. Only the MAPS-based device responded to oxLDL and the sensing layer was insensitive to other serum proteins, such as myoglobin and haemoglobin. Linear behaviour between log(C, μg mL−1) versus charged tranfer resistance (RCT, Ω) was observed by electrochemical impedance spectroscopy (EIS). Calibrations made in Fetal Calf Serum (FCS) were linear from 2.5 to 12.5 μg mL−1 (RCT = 946.12 × log C + 1590.7) with an R-squared of 0.9966. Overall, these were promising results towards the design of materials acting close to the natural antibodies and applied to practical use of clinical interest.

Graphical abstract: Backside-surface imprinting as a new strategy to generate specific plastic antibody materials

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Publication details

The article was received on 07 Dec 2013, accepted on 25 Feb 2014 and first published on 05 Mar 2014


Article type: Paper
DOI: 10.1039/C3TB21740J
Citation: J. Mater. Chem. B, 2014,2, 3087-3095
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    Backside-surface imprinting as a new strategy to generate specific plastic antibody materials

    G. Cabral-Miranda, M. Gidlund and M. G. F. Sales, J. Mater. Chem. B, 2014, 2, 3087
    DOI: 10.1039/C3TB21740J

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