Issue 17, 2014

Mitochondrial targeting dendrimer allows efficient and safe gene delivery

Abstract

Cationic dendrimers are promising tools in the introduction of nucleic acids into mammalian cells, however, present dendrimer-based gene vectors have modest transfection efficacy and significant toxicity. Here, we synthesized triphenylphosphonium (TPP) conjugated dendrimers and used them as polymeric gene vectors. TPP-conjugated dendrimers can effectively target mitochondria and showed efficient transfection efficacy on HeLa and COS-7 cells with low cytotoxicity on the transfected cells. These materials achieved much higher efficacy than the commercial transfection reagent SuperFect and comparable efficacy with Lipofectamine 2000. This study provides a new insight into the design of efficient and biocompatible gene vectors using dendrimers as scaffolds.

Graphical abstract: Mitochondrial targeting dendrimer allows efficient and safe gene delivery

Supplementary files

Article information

Article type
Paper
Submitted
29 Sep 2013
Accepted
22 Nov 2013
First published
28 Nov 2013

J. Mater. Chem. B, 2014,2, 2546-2553

Mitochondrial targeting dendrimer allows efficient and safe gene delivery

X. Wang, N. Shao, Q. Zhang and Y. Cheng, J. Mater. Chem. B, 2014, 2, 2546 DOI: 10.1039/C3TB21348J

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