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Issue 37, 2014
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Trehalose facilitates DNA melting: a single-molecule optical tweezers study

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Abstract

Using optical tweezers, here we show that the overstretching transition force of double-stranded DNA (dsDNA) is lowered significantly by the addition of the disaccharide trehalose as well as certain polyol osmolytes. This effect is found to depend linearly on the logarithm of the trehalose concentration. We propose an entropic driving mechanism for the experimentally observed destabilization of dsDNA that is rooted in the higher affinity of the DNA bases for trehalose than for water, which promotes base exposure and DNA melting. Molecular dynamics simulation reveals the direct interaction of trehalose with nucleobases. Experiments with other osmolytes confirm that the extent of dsDNA destabilization is governed by the ratio between polar and apolar fractions of an osmolyte.

Graphical abstract: Trehalose facilitates DNA melting: a single-molecule optical tweezers study

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Publication details

The article was received on 12 Jul 2014, accepted on 25 Jul 2014 and first published on 06 Aug 2014


Article type: Paper
DOI: 10.1039/C4SM01532K
Citation: Soft Matter, 2014,10, 7269-7277
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    Trehalose facilitates DNA melting: a single-molecule optical tweezers study

    S. Bezrukavnikov, A. Mashaghi, R. J. van Wijk, C. Gu, L. J. Yang, Y. Q. Gao and S. J. Tans, Soft Matter, 2014, 10, 7269
    DOI: 10.1039/C4SM01532K

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