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Issue 40, 2014
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3D Viscoelastic traction force microscopy

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Abstract

Native cell–material interactions occur on materials differing in their structural composition, chemistry, and physical compliance. While the last two decades have shown the importance of traction forces during cell–material interactions, they have been almost exclusively presented on purely elastic in vitro materials. Yet, most bodily tissue materials exhibit some level of viscoelasticity, which could play an important role in how cells sense and transduce tractions. To expand the realm of cell traction measurements and to encompass all materials from elastic to viscoelastic, this paper presents a general, and comprehensive approach for quantifying 3D cell tractions in viscoelastic materials. This methodology includes the experimental characterization of the time-dependent material properties for any viscoelastic material with the subsequent mathematical implementation of the determined material model into a 3D traction force microscopy (3D TFM) framework. Utilizing this new 3D viscoelastic TFM (3D VTFM) approach, we quantify the influence of viscosity on the overall material traction calculations and quantify the error associated with omitting time-dependent material effects, as is the case for all other TFM formulations. We anticipate that the 3D VTFM technique will open up new avenues of cell–material investigations on even more physiologically relevant time-dependent materials including collagen and fibrin gels.

Graphical abstract: 3D Viscoelastic traction force microscopy

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Publication details

The article was received on 12 Jun 2014, accepted on 14 Aug 2014 and first published on 29 Aug 2014


Article type: Paper
DOI: 10.1039/C4SM01271B
Citation: Soft Matter, 2014,10, 8095-8106
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    3D Viscoelastic traction force microscopy

    J. Toyjanova, E. Hannen, E. Bar-Kochba, E. M. Darling, D. L. Henann and C. Franck, Soft Matter, 2014, 10, 8095
    DOI: 10.1039/C4SM01271B

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