Ambient condition oxidation in individual liposomes observed at the single molecule level

Abstract

Single particle imaging studies on liposomes stained with lipophilic, fluorogenic, peroxyl radical-trapping probes show oxidation taking place during standard liposome preparation conditions. A ratiometric assay involving the fluorogenic probe enables quantifying oxidative events at the single particle level. The assay provides a general strategy to monitor reactions on individual liposomes.