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Issue 12, 2014
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Enzymatic self-sacrificial display of an active protein on gold nanoparticles

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Abstract

A new enzymatic approach for the fabrication of gold nanoparticles (Au NPs) decorated with a functional protein was developed using a recombinant glycerol dehydrogenase from Bacillus stearothermophilus (BsGLD) that was tagged with an N-terminal protein G domain and a C-terminal (His)6Cys tag. The recombinant BsGLD promoted the synthesis of the Au NPs via the catalytic reduction of nicotinamide adenine dinucleotide using glycerol as a substrate, and the recombinant BsGLD was self-immobilized on the Au NPs as a result of the protein G's binding capability for IgG antibody.

Graphical abstract: Enzymatic self-sacrificial display of an active protein on gold nanoparticles

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Publication details

The article was received on 04 Nov 2013, accepted on 16 Dec 2013 and first published on 09 Jan 2014


Article type: Communication
DOI: 10.1039/C3RA46384B
Citation: RSC Adv., 2014,4, 5995-5998
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    Enzymatic self-sacrificial display of an active protein on gold nanoparticles

    T. Niide, M. Goto and N. Kamiya, RSC Adv., 2014, 4, 5995
    DOI: 10.1039/C3RA46384B

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