Jump to main content
Jump to site search

Issue 94, 2014
Previous Article Next Article

Real-time detection of telomerase activity in cancer cells using a label-free electrochemical impedimetric biosensing microchip

Author affiliations

Abstract

The enzyme telomerase is present in about 85% of human cancers which makes it not only a good target for cancer treatment but also an excellent marker for cancer detection. Using a single stranded DNA probe specific for telomerase binding and reverse transcription tethered to an interdigital gold electrode array surface, the chromosome protection provided by the telomerase was replicated and followed by electrochemical impedance spectroscopy as an unlabeled biosensor. Using this system designed in-house, easy and affordable, impedance measurements were taken while incubating at 37 °C and promoting the probe elongation. This resulted in up to 14-fold increase in the charge transfer resistance when testing a telomerase-positive nuclear extract from Jurkat cells compared to the heat-inactivated telomerase-negative nuclear extract. The electron transfer process at the Au electrodes was studied before the elongation, at different times after the elongation, and after desorption of non-specific binding.

Graphical abstract: Real-time detection of telomerase activity in cancer cells using a label-free electrochemical impedimetric biosensing microchip

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Sep 2014, accepted on 13 Oct 2014 and first published on 15 Oct 2014


Article type: Paper
DOI: 10.1039/C4RA09689D
Author version available: Download Author version (PDF)
Citation: RSC Adv., 2014,4, 52357-52365
  •   Request permissions

    Real-time detection of telomerase activity in cancer cells using a label-free electrochemical impedimetric biosensing microchip

    L. Cunci, M. Martinez Vargas, R. Cunci, R. Gomez-Moreno, I. Perez, A. Baerga-Ortiz, C. I. Gonzalez and C. R. Cabrera, RSC Adv., 2014, 4, 52357
    DOI: 10.1039/C4RA09689D

Search articles by author

Spotlight

Advertisements