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Issue 94, 2014
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Diazo group as a new chemical reporter for bioorthogonal labelling of biomolecules

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Abstract

Diazoacetyl groups have been shown to undergo spontaneous cycloaddition reactions with strained alkenes and alkynes. The rates of reaction are similar to the equivalent reactions of azide groups. This allows diazo groups to be used as bioorthogonal reporter groups. This is demonstrated by fluorescent labelling of a diazoacetylated protein and of cell-surface glycans via metabolic incorporation of a diazoacetylated sugar.

Graphical abstract: Diazo group as a new chemical reporter for bioorthogonal labelling of biomolecules

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Publication details

The article was received on 18 Aug 2014, accepted on 13 Oct 2014 and first published on 14 Oct 2014


Article type: Paper
DOI: 10.1039/C4RA08861A
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Citation: RSC Adv., 2014,4, 52241-52244
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    Diazo group as a new chemical reporter for bioorthogonal labelling of biomolecules

    L. Josa-Culleré, Y. A. Wainman, K. M. Brindle and F. J. Leeper, RSC Adv., 2014, 4, 52241
    DOI: 10.1039/C4RA08861A

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