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Issue 83, 2014
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Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH

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Abstract

We report a novel method for studying cellular migration in vitro. Cytophilic microdomains were formed on a cytophobic substrate by laser ablation. HeLa cells were grown on those domains until confluence, and then channels were formed to guide cellular migration. Two-photon excitation fluorescence-lifetime imaging of NADH revealed metabolic variation among migrating and nonmigrating cells.

Graphical abstract: Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH

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Publication details

The article was received on 01 Jul 2014, accepted on 09 Sep 2014 and first published on 17 Sep 2014


Article type: Communication
DOI: 10.1039/C4RA06492E
Author version available: Download Author version (PDF)
Citation: RSC Adv., 2014,4, 44100-44104
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    Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH

    G. Deka, K. Okano, H. Masuhara, Y. Li and F. Kao, RSC Adv., 2014, 4, 44100
    DOI: 10.1039/C4RA06492E

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