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Issue 31, 2014
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An automated materials screening approach for the development of sol–gel derived monolithic silica enzyme reactor columns

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Abstract

Fabrication of monolithic protein-doped capillary columns was reported almost 10 years ago. These columns were derived from a diglycerylsilane precursor, however this material is not commercially available, is difficult to produce in large quantities and has very short phase separation and gelation times, which leads to issues with column reproducibility. Herein, we investigate the use of sodium silicate (SS), a commercially available biofriendly sol–gel precursor, for the fabrication of bimodal meso/macroporous protein-doped monolithic silica columns that are suitable for immobilized enzyme reactor (IMER) assays. Using an automated liquid handler and platereader, a hierarchical materials screening approach was applied to ∼1400 formulations, from which we have identified materials with long gelation times that can form robust bimodal meso/macroporous materials suitable for fabrication of monolithic silica columns. A subset of these materials was observed to have good chromatographic behavior (appropriate backpressure and good stability). A secondary screen around lead materials was performed to identify optimal materials for fabrication of IMER columns. These materials were tested for leaching and activity of immobilized acetylcholine esterase to identify an optimal material for IMER column fabrication. The optimal material was formed from 2% (w/v) silica which was combined with 1.25% PEG 600 at pH 6.4 in 100 mM TRIS buffer. Such columns showed reproducible IMER performance and were able to quantitatively measure the inhibition of immobilized AChE by galanthamine with an inhibition constant of 175 ± 5 nM, which is in excellent agreement with the literature value.

Graphical abstract: An automated materials screening approach for the development of sol–gel derived monolithic silica enzyme reactor columns

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Publication details

The article was received on 24 Jan 2014, accepted on 18 Mar 2014 and first published on 28 Mar 2014


Article type: Paper
DOI: 10.1039/C4RA00734D
Citation: RSC Adv., 2014,4, 15952-15960
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    An automated materials screening approach for the development of sol–gel derived monolithic silica enzyme reactor columns

    A. M. E. Smith, J. Fortuna, E. M. Forsberg and J. D. Brennan, RSC Adv., 2014, 4, 15952
    DOI: 10.1039/C4RA00734D

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