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Issue 14, 2014
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Engineered fluorescence tags for in vivo protein labelling

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Abstract

In vivo protein labelling with a peptide tag–fluorescent probe system is an important chemical biology strategy for studying protein distribution, interaction and function. A variety of engineered tags have been developed to label proteins of interest (POIs), in which a protein or peptide tag is covalently linked to the POIs that can afford fluorescence signal under certain conditions. This review summarizes the current methodology in five categories, namely autofluorescent protein tags, peptide tags with a specific probe-binding amino acid sequence, peptide tags that can be further labelled by enzyme recognition, peptide tags that contain an enzymatic domain which can interact with small molecules or coenzymes, and peptide tags that can be labelled with probes through natural or artificial protein–protein interaction. The principles and application of each tool are also reviewed.

Graphical abstract: Engineered fluorescence tags for in vivo protein labelling

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Publication details

The article was received on 25 Nov 2013, accepted on 06 Jan 2014 and first published on 07 Jan 2014


Article type: Review Article
DOI: 10.1039/C3RA46991C
Citation: RSC Adv., 2014,4, 7235-7245
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    Engineered fluorescence tags for in vivo protein labelling

    Z. Wang, X. Ding, S. Li, J. Shi and Y. Li, RSC Adv., 2014, 4, 7235
    DOI: 10.1039/C3RA46991C

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