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Issue 6, 2014
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Aggregation/disaggregation of chlorophyll a in model phospholipid–detergent vesicles and micelles

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Abstract

The photosynthetic pigments of higher plants exist in complex oligomeric states, which are difficult to study in vivo. To investigate aggregation processes of chlorophyll a (Chl a), we used an in vitro reconstitution procedure, with this pigment incorporated into liposomes of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), micelles and pre-micelle media of the detergent n-dodecyltrimethylammonium chloride (DTAC), and mixed, spontaneous, DMPC–DTAC vesicles and micelles. Chl a oligomers were characterized by UV-visible absorption, steady-state and time-resolved fluorescence, and fluorescence lifetime imaging microscopy. Equivalent diameters of the colloidal structures were obtained by fluorescence correlation spectroscopy. In DMPC liposomes and DMPC–DTAC vesicles and micelles, three fluorescence lifetimes indicated the coexistence of Chl a monomers (≈5 ns) and oligomers (≈1–2 to ≈0.1 ns). The increase in DTAC amount, in the mixed system, induces a progressive solubilization of DMPC liposomes (from vesicles to micelles) and simultaneous disruption of Chl a aggregates; in pure DTAC micelles, mostly monomers were found. The present work aims for a better understanding of chlorophyll–chlorophyll (Chl–Chl), Chl–lipid, and Chl–detergent interactions in spontaneous colloidal micro- and nanostructures.

Graphical abstract: Aggregation/disaggregation of chlorophyll a in model phospholipid–detergent vesicles and micelles

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Publication details

The article was received on 05 Dec 2013, accepted on 02 Mar 2014 and first published on 05 Mar 2014


Article type: Paper
DOI: 10.1039/C3PP50419K
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Citation: Photochem. Photobiol. Sci., 2014,13, 907-916
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    Aggregation/disaggregation of chlorophyll a in model phospholipid–detergent vesicles and micelles

    R. F. Correia, M. I. Viseu and S. M. Andrade, Photochem. Photobiol. Sci., 2014, 13, 907
    DOI: 10.1039/C3PP50419K

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