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Issue 10, 2014
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Synthesis, structure and inhibitory activity of a stereoisomer of oseltamivir carboxylate

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Abstract

A stereodivergent plan is presented leading to all eight stereoisomers of oseltamivir carboxylate (OC). Key chemical manoeuvers are (1) a three-component vinylogous Mukaiyama–Mannich reaction, which sets the whole carbon skeleton and heteroatom substituents, and (2) an intramolecular, silylative Mukaiyama aldol reaction, which creates the targeted carbocycle. The viability of the plan was demonstrated by the first total synthesis of 4-epi-oseltamivir carboxylate (6), accessed in 15 steps from glyceraldehyde, o-anisidine and pyrrole siloxydiene precursors. Compound 6 inhibits influenza A virus strains H1N1 and H3N2 at the μM level, about 150 000-fold less than the OC reference, testifying that the stereodisposition of the C4 acetamido function is key for enzyme recognition. Guided by in-depth structural evaluation including NMR solution studies, molecular mechanics simulations, docking analyses and X-ray crystallography, rationalization of the biological verdict was established.

Graphical abstract: Synthesis, structure and inhibitory activity of a stereoisomer of oseltamivir carboxylate

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Publication details

The article was received on 17 Oct 2013, accepted on 21 Nov 2013 and first published on 22 Nov 2013


Article type: Paper
DOI: 10.1039/C3OB42069H
Citation: Org. Biomol. Chem., 2014,12, 1561-1569
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    Synthesis, structure and inhibitory activity of a stereoisomer of oseltamivir carboxylate

    A. Sartori, L. Dell'Amico, L. Battistini, C. Curti, S. Rivara, D. Pala, P. S. Kerry, G. Pelosi, G. Casiraghi, G. Rassu and F. Zanardi, Org. Biomol. Chem., 2014, 12, 1561
    DOI: 10.1039/C3OB42069H

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