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Issue 27, 2014
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A novel chemosensor with visible light excitability for sensing Zn2+ in physiological medium and in HeLa cells

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Abstract

In the present study a novel imine-hydrazone based fluorescent chemosensor (L1) for efficient and selective sensing of Zn2+ over other biologically important metal ions under physiological conditions is reported. An enhancement in fluorescence emission intensity of the developed probe with a red shift of ∼25 nm was observed for Zn2+, whereas other metal ions failed to reveal any significant change in the emission spectra. Interestingly, the receptor functioned under completely physiological conditions (99.7% HEPES buffer) and has visible light excitability. Sensing of Zn2+ was investigated in detail by absorption spectroscopy, emission spectroscopy, DFT calculation, 1H-NMR titration experiment and ESI-MS experiment. The association constant between L1 and Zn2+ was found to be 5.58 × 105 M−1. The receptor could detect as low as 69 ppb Zn2+. Sensing of Zn2+ is proposed through switch-on of intramolecular charge transfer (ICT) and chelation enhanced fluorescence (CHEF) processes after the introduction of Zn2+ into the free ligand. The developed receptor was non-toxic and rendered intracellular sensing of Zn2+ in HeLa cells through fluorescence imaging studies.

Graphical abstract: A novel chemosensor with visible light excitability for sensing Zn2+ in physiological medium and in HeLa cells

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Publication details

The article was received on 27 Mar 2014, accepted on 13 May 2014 and first published on 13 May 2014


Article type: Paper
DOI: 10.1039/C4OB00653D
Author version available: Download Author version (PDF)
Citation: Org. Biomol. Chem., 2014,12, 4975-4982
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    A novel chemosensor with visible light excitability for sensing Zn2+ in physiological medium and in HeLa cells

    B. K. Datta, D. Thiyagarajan, S. Samanta, A. Ramesh and G. Das, Org. Biomol. Chem., 2014, 12, 4975
    DOI: 10.1039/C4OB00653D

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