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β-Amyrin synthase from Euphorbia tirucalli. Steric bulk, not the π-electrons of Phe, at position 474 has a key role in affording the correct folding of the substrate to complete the normal polycyclization cascade

Corresponding authors
Graduate School of Science and Technology, and Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata 950-2181, Japan
Org. Biomol. Chem., 2014,12, 3836-3846

DOI: 10.1039/C4OB00064A
Received 09 Jan 2014, Accepted 14 Feb 2014
First published online 17 Feb 2014

This article is part of themed collection: 2014 Hot Articles in Organic and Biomolecular Chemistry
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