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β-Amyrin synthase from Euphorbia tirucalli. Steric bulk, not the π-electrons of Phe, at position 474 has a key role in affording the correct folding of the substrate to complete the normal polycyclization cascade

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Graduate School of Science and Technology, and Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata 950-2181, Japan
E-mail: hoshitsu@agr.niigata-u.ac.jp
Org. Biomol. Chem., 2014, Advance Article

DOI: 10.1039/C4OB00064A
Received 09 Jan 2014, Accepted 14 Feb 2014
First published online 17 Feb 2014
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