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Issue 19, 2014
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A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism

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Abstract

An ADP selective fluorescent probe working in aqueous medium was identified and the change in fluorescence as a function of ADP concentration was standardized. Using this probe, all the steps of glycolysis coupled with ATP/ADP inter-conversion and oxidative breakdown of pyruvate in the mitochondria were monitored and the consumption/production of ATP/ADP at each step was quantified. The quantity of ADP present in the mitochondria, taken from different body parts of a pig, was also determined. It is hypothesized that an appropriate modification of the technique may provide a diagnostic tool for monitoring biochemical pathways as well as for quick estimation of ADP in the mitochondria and other cell organelles.

Graphical abstract: A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism

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Publication details

The article was received on 14 Dec 2013, accepted on 04 Mar 2014 and first published on 04 Mar 2014


Article type: Paper
DOI: 10.1039/C3OB42505C
Citation: Org. Biomol. Chem., 2014,12, 3071-3079
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    A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism

    A. Kumar, P. Prasher and P. Singh, Org. Biomol. Chem., 2014, 12, 3071
    DOI: 10.1039/C3OB42505C

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