Issue 19, 2014

A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism

Abstract

An ADP selective fluorescent probe working in aqueous medium was identified and the change in fluorescence as a function of ADP concentration was standardized. Using this probe, all the steps of glycolysis coupled with ATP/ADP inter-conversion and oxidative breakdown of pyruvate in the mitochondria were monitored and the consumption/production of ATP/ADP at each step was quantified. The quantity of ADP present in the mitochondria, taken from different body parts of a pig, was also determined. It is hypothesized that an appropriate modification of the technique may provide a diagnostic tool for monitoring biochemical pathways as well as for quick estimation of ADP in the mitochondria and other cell organelles.

Graphical abstract: A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism

Supplementary files

Article information

Article type
Paper
Submitted
14 Dec 2013
Accepted
04 Mar 2014
First published
04 Mar 2014

Org. Biomol. Chem., 2014,12, 3071-3079

A fluorescent probe for estimation of adenosine diphosphate and monitoring of glucose metabolism

A. Kumar, P. Prasher and P. Singh, Org. Biomol. Chem., 2014, 12, 3071 DOI: 10.1039/C3OB42505C

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