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Issue 12, 2014
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Amphiphilic polyethylenimine (PEI) as highly efficient non-viral gene carrier

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Abstract

Efficient and safe gene vectors are important for gene therapy. Here, a novel family of amphiphilic polyethylenimine (PEI) LD1-PEI bearing a polar group of branched PEI 25K and four dodecyl chains was developed. Agarose gel electrophoresis was used to confirm the formation of complexes. The transfection activity of the amphiphilic carrier was evaluated in different cell lines. The in vitro study showed that LD1-PEI showed a higher transfection efficiency with improved biocompatibility than PEI 25K. Serum showed almost no or only a slight effect on LD1-PEI/DNA transfection efficiency. In summary, LD1-PEI is a promising nonviral gene carrier.

Graphical abstract: Amphiphilic polyethylenimine (PEI) as highly efficient non-viral gene carrier

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Publication details

The article was received on 15 Nov 2013, accepted on 13 Dec 2013 and first published on 16 Dec 2013


Article type: Paper
DOI: 10.1039/C3OB42279H
Citation: Org. Biomol. Chem., 2014,12, 1975-1982
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    Amphiphilic polyethylenimine (PEI) as highly efficient non-viral gene carrier

    X. Yan, Y. Zhang, H. Zhang, P. G. Wang, X. Chu and X. Wang, Org. Biomol. Chem., 2014, 12, 1975
    DOI: 10.1039/C3OB42279H

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