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Issue 14, 2014
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Rapid fibroblast activation in mammalian cells induced by silicon nanowire arrays

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Abstract

Activated tumor-associated fibroblasts (TAFs) with abundant fibroblast activation protein (FAP) expression attract tremendous attention in tumor progression studies. In this work, we report a rapid 24 h FAP activation method for fibroblasts using silicon nanowires (SiNWs) as culture substrates instead of growth factors or chemokines. In contrast with cells cultured on flat silicon which rarely express FAP, SiNW cultivated cells exhibit FAP levels similar to those found in cancerous tissue. We demonstrated that activated cells grown on SiNWs maintain their viability and proliferation in a time-dependent manner. Moreover, environmental scanning electron microscopy (ESEM) and focused ion beam and scanning electron microscopy (FIB-SEM) analysis clearly revealed that activated cells on SiNWs adapt to the structure of their substrates by filling inter-wire cavities via filopodia in contrast to cells cultured on flat silicon which spread freely. We further illustrated that the expression of FAP was rarely detected in activated cells after being re-cultured in Petri dishes, suggesting that the unique structure of SiNWs may have a certain influence on FAP activation.

Graphical abstract: Rapid fibroblast activation in mammalian cells induced by silicon nanowire arrays

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Publication details

The article was received on 14 Mar 2014, accepted on 03 May 2014 and first published on 06 May 2014


Article type: Paper
DOI: 10.1039/C4NR01415D
Citation: Nanoscale, 2014,6, 8318-8325
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    Rapid fibroblast activation in mammalian cells induced by silicon nanowire arrays

    Q. Ha, G. Yang, Z. Ao, D. Han, F. Niu and S. Wang, Nanoscale, 2014, 6, 8318
    DOI: 10.1039/C4NR01415D

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