Jump to main content
Jump to site search

Issue 13, 2014
Previous Article Next Article

Untying a nanoscale knotted polymer structure to linear chains for efficient gene delivery in vitro and to the brain

Author affiliations

Abstract

The purpose of this study was to develop a platform transfection technology, for applications in the brain, which could transfect astrocytes without requiring cell specific functionalization and without the common cause of toxicity through high charge density. Here we show that a simple and scalable preparation technique can be used to produce a “knot” structured cationic polymer, where single growing chains can crosslink together via disulphide intramolecular crosslinks (internal cyclizations). This well-defined knot structure can thus “untie” under reducing conditions, showing a more favorable transfection profile for astrocytes compared to 25 kDa-PEI (48-fold), SuperFect® (39-fold) and Lipofectamine®2000 (18-fold) whilst maintaining neural cell viability at over 80% after four days of culture. The high transfection/lack of toxicity of this knot structured polymer in vitro, combined with its ability to mediate luciferase transgene expression in the adult rat brain, demonstrates its use as a platform transfection technology which should be investigated further for neurodegenerative disease therapies.

Graphical abstract: Untying a nanoscale knotted polymer structure to linear chains for efficient gene delivery in vitro and to the brain

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Dec 2013, accepted on 15 Apr 2014 and first published on 17 Apr 2014


Article type: Paper
DOI: 10.1039/C3NR06737H
Author version available: Download Author version (PDF)
Citation: Nanoscale, 2014,6, 7526-7533
  •   Request permissions

    Untying a nanoscale knotted polymer structure to linear chains for efficient gene delivery in vitro and to the brain

    B. Newland, A. Aied, A. V. Pinoncely, Y. Zheng, T. Zhao, H. Zhang, R. Niemeier, E. Dowd, A. Pandit and W. Wang, Nanoscale, 2014, 6, 7526
    DOI: 10.1039/C3NR06737H

Search articles by author

Spotlight

Advertisements