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Issue 4, 2014
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Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

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Abstract

Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and ‘on–off’ switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition.

Graphical abstract: Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

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Publication details

The article was received on 16 Sep 2013, accepted on 04 Nov 2013 and first published on 05 Nov 2013


Article type: Paper
DOI: 10.1039/C3NR04952C
Citation: Nanoscale, 2014,6, 2368-2374
  • Open access: Creative Commons BY license
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    Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

    J. P. Magnusson, F. Fernández-Trillo, G. Sicilia, S. G. Spain and C. Alexander, Nanoscale, 2014, 6, 2368
    DOI: 10.1039/C3NR04952C

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