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Issue 3, 2014
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On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

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Abstract

Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.

Graphical abstract: On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

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Publication details

The article was received on 20 Aug 2013, accepted on 20 Nov 2013 and first published on 25 Nov 2013


Article type: Paper
DOI: 10.1039/C3NR04432G
Citation: Nanoscale, 2014,6, 1741-1747
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    On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

    H. Deschout, K. Raemdonck, S. Stremersch, P. Maoddi, G. Mernier, P. Renaud, S. Jiguet, A. Hendrix, M. Bracke, R. Van den Broecke, M. Röding, M. Rudemo, J. Demeester, S. C. De Smedt, F. Strubbe, K. Neyts and K. Braeckmans, Nanoscale, 2014, 6, 1741
    DOI: 10.1039/C3NR04432G

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