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Issue 3, 2014
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A bidirectional fluorescent two-hybrid system for monitoring protein–protein interactions

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Abstract

Two-hybrid systems have been the cornerstone of research into protein–protein interactions, but these systems typically rely on life/death reporters that put additional selective pressure on the host organism, and potentially lead to false positives. Here we report a bidirectional fluorescence-based bacterial two-hybrid system that enables both the association and dissociation of a given protein–protein interaction to be monitored. The functionality of this system and its compatibility with FACS screening are demonstrated in the forward and reverse direction using known interacting protein-partners and their cyclic peptide inhibitors. The reported fluorescent two-hybrid system may be used in the forward direction for the identification of interacting protein partners, or as a reverse two-hybrid system for the high-throughput identification of protein–protein interaction inhibitors.

Graphical abstract: A bidirectional fluorescent two-hybrid system for monitoring protein–protein interactions

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Publication details

The article was received on 30 Sep 2013, accepted on 29 Nov 2013 and first published on 29 Nov 2013


Article type: Paper
DOI: 10.1039/C3MB70438F
Citation: Mol. BioSyst., 2014,10, 485-490
  • Open access: Creative Commons BY-NC license
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    A bidirectional fluorescent two-hybrid system for monitoring protein–protein interactions

    I. K. Nordgren and A. Tavassoli, Mol. BioSyst., 2014, 10, 485
    DOI: 10.1039/C3MB70438F

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