Jump to main content
Jump to site search

Issue 2, 2014
Previous Article Next Article

Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1

Author affiliations

Abstract

Fluorescent protein (FP)-based biosensors have revolutionized the ability of researchers to monitor enzyme activities in live cells. While the basic design principles for FP-based biosensors are well established, first-generation biosensor constructs typically suffer from relatively low fluorescence responses that limit their general applicability. The protein engineering efforts required to substantially improve the biosensor responses are often both labour and time intensive. Here we report the application of a high throughput bacterial colony screen for improving the response of kinase biosensors. This effort led to the development of a second-generation cyclin B1-CDK1 biosensor with a 4.5-fold greater response than the first-generation biosensor.

Graphical abstract: Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 Sep 2013, accepted on 16 Nov 2013 and first published on 19 Nov 2013


Article type: Communication
DOI: 10.1039/C3MB70402E
Citation: Mol. BioSyst., 2014,10, 191-195
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1

    A. S. F. Belal, B. R. Sell, H. Hoi, M. W. Davidson and R. E. Campbell, Mol. BioSyst., 2014, 10, 191
    DOI: 10.1039/C3MB70402E

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements