Jump to main content
Jump to site search

Issue 11, 2014
Previous Article Next Article

A metabolic trade-off between phosphate and glucose utilization in Escherichia coli

Author affiliations

Abstract

Getting the most out of available nutrients is a key challenge that all organisms face. Little is known about how they optimize and balance the simultaneous utilization of multiple elemental resources. We investigated the effects of long-term phosphate limitation on carbon metabolism of the model organism Escherichia coli using chemostat cultures. We profiled metabolic changes in the growth medium over time and found evidence for an increase in fermentative metabolism despite the aerobic conditions. Using full-genome sequencing and competition experiments, we found that fitness under phosphate-limiting conditions was reproducibly increased by a mutation preventing flux through succinate in the tricarboxylic acid cycle. In contrast, these mutations reduced competitive ability under carbon limitation, and thus reveal a conflicting metabolic benefit in the role of the TCA cycle in environments limited by inorganic phosphate and glucose.

Graphical abstract: A metabolic trade-off between phosphate and glucose utilization in Escherichia coli

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 May 2014, accepted on 08 Aug 2014 and first published on 11 Aug 2014


Article type: Communication
DOI: 10.1039/C4MB00313F
Author version available: Download Author version (PDF)
Citation: Mol. BioSyst., 2014,10, 2820-2822
  •   Request permissions

    A metabolic trade-off between phosphate and glucose utilization in Escherichia coli

    V. Behrends, R. P. Maharjan, B. Ryall, L. Feng, B. Liu, L. Wang, J. G. Bundy and T. Ferenci, Mol. BioSyst., 2014, 10, 2820
    DOI: 10.1039/C4MB00313F

Search articles by author

Spotlight

Advertisements