Jump to main content
Jump to site search

Issue 7, 2014
Previous Article Next Article

A comprehensive analysis of the Streptococcus pyogenes and human plasma protein interaction network

Author affiliations

Abstract

Streptococcus pyogenes is a major human bacterial pathogen responsible for severe and invasive disease associated with high mortality rates. The bacterium interacts with several human blood plasma proteins and clarifying these interactions and their biological consequences will help to explain the progression from mild to severe infections. In this study, we used a combination of mass spectrometry (MS) based techniques to comprehensively quantify the components of the S. pyogenes–plasma protein interaction network. From an initial list of 181 interacting human plasma proteins defined using liquid chromatography (LC)-MS/MS analysis we further subdivided the interacting protein list using selected reaction monitoring (SRM) depending on the level of enrichment and protein concentration on the bacterial surface. The combination of MS methods revealed several previously characterized interactions between the S. pyogenes surface and human plasma along with many more, so far uncharacterised, possible plasma protein interactions with S. pyogenes. In follow-up experiments, the combination of MS techniques was applied to study differences in protein binding to a S. pyogenes wild type strain and an isogenic mutant lacking several important virulence factors, and a unique pair of invasive and non-invasive S. pyogenes isolates from the same patient. Comparing the plasma protein-binding properties of the wild type and the mutant and the invasive and non-invasive S. pyogenes bacteria revealed considerable differences, underlining the significance of these protein interactions. The results also demonstrate the power of the developed mass spectrometry method to investigate host–microbial relationships with a large proteomics depth and high quantitative accuracy.

Graphical abstract: A comprehensive analysis of the Streptococcus pyogenes and human plasma protein interaction network

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Dec 2013, accepted on 25 Jan 2014 and first published on 28 Jan 2014


Article type: Paper
DOI: 10.1039/C3MB70555B
Author version available: Download Author version (PDF)
Citation: Mol. BioSyst., 2014,10, 1698-1708
  •   Request permissions

    A comprehensive analysis of the Streptococcus pyogenes and human plasma protein interaction network

    K. Sjöholm, C. Karlsson, A. Linder and J. Malmström, Mol. BioSyst., 2014, 10, 1698
    DOI: 10.1039/C3MB70555B

Search articles by author

Spotlight

Advertisements