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Issue 17, 2014
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A light-sheet microscope compatible with mobile devices for label-free intracellular imaging and biosensing

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Abstract

The inner structure, especially the nuclear structure, of cells carries valuable information about disease and health conditions of a person. Here we demonstrate a label-free technique to enable direct observations and measurements of the size, shape and morphology of the cell nucleus. With a microfabricated lens and a commercial CMOS imager, we form a scanning light-sheet microscope to produce a dark-field optical scattering image of the cell nucleus that overlays with the bright-field image produced in a separate regime of the same CMOS sensor. We have used the device to detect nuclear features that characterize the life cycle of cells and have used the nucleus volume as a new parameter for cell classification. The device can be developed into a portable, low-cost, point-of-care device leveraging the capabilities of the CMOS imagers to be pervasive in mobile electronics.

Graphical abstract: A light-sheet microscope compatible with mobile devices for label-free intracellular imaging and biosensing

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Publication details

The article was received on 28 Feb 2014, accepted on 20 May 2014 and first published on 20 May 2014


Article type: Paper
DOI: 10.1039/C4LC00257A
Citation: Lab Chip, 2014,14, 3341-3348
  • Open access: Creative Commons BY license
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    A light-sheet microscope compatible with mobile devices for label-free intracellular imaging and biosensing

    T. Wu, T. M. Yen, Y. Han, Y. Chiu, E. Y.-S. Lin and Y. Lo, Lab Chip, 2014, 14, 3341
    DOI: 10.1039/C4LC00257A

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