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Issue 23, 2014
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An osmolyte-based micro-volume ultrafiltration technique

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Abstract

This paper discusses a novel, simple, and inexpensive micro-volume ultrafiltration technique for protein concentration, desalting, buffer exchange, and size-based protein purification. The technique is suitable for processing protein samples in a high-throughput mode. It utilizes a combination of capillary action, and osmosis for drawing water and other permeable species from a micro-volume sample droplet applied on the surface of an ultrafiltration membrane. A macromolecule coated on the permeate side of the membrane functions as the osmolyte. The action of the osmolyte could, if required, be augmented by adding a supersorbent polymer layer over the osmolyte. The mildly hydrophobic surface of the polymeric ultrafiltration membrane used in this study minimized sample droplet spreading, thus making it easy to recover the retained material after separation, without sample interference and cross-contamination. High protein recoveries were observed in the micro-volume ultrafiltration experiments described in the paper.

Graphical abstract: An osmolyte-based micro-volume ultrafiltration technique

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Publication details

The article was received on 31 Aug 2014, accepted on 29 Sep 2014 and first published on 29 Sep 2014


Article type: Paper
DOI: 10.1039/C4LC01014K
Citation: Lab Chip, 2014,14, 4559-4566
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    An osmolyte-based micro-volume ultrafiltration technique

    R. Ghosh, Lab Chip, 2014, 14, 4559
    DOI: 10.1039/C4LC01014K

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