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Issue 23, 2014
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Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems

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Abstract

Picoinjection is a promising technique to add reagents into pre-formed emulsion droplets on chip however, it is sensitive to pressure fluctuation, making stable operation of the picoinjector challenging. We present a chip architecture using a simple pressure stabilizer for consistent and highly reproducible picoinjection in multi-step biochemical assays with droplets. Incorporation of the stabilizer immediately upstream of a picoinjector or a combination of injectors greatly reduces pressure fluctuations enabling reproducible and effective picoinjection in systems where the pressure varies actively during operation. We demonstrate the effectiveness of the pressure stabilizer for an integrated platform for on-demand encapsulation of bacterial cells followed by picoinjection of reagents for lysing the encapsulated cells. The pressure stabilizer was also used for picoinjection of multiple displacement amplification (MDA) reagents to achieve genomic DNA amplification of lysed bacterial cells.

Graphical abstract: Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems

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Publication details

The article was received on 15 Jul 2014, accepted on 18 Sep 2014 and first published on 30 Sep 2014


Article type: Paper
DOI: 10.1039/C4LC00823E
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2014,14, 4533-4539
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    Pressure stabilizer for reproducible picoinjection in droplet microfluidic systems

    M. Rhee, Y. K. Light, S. Yilmaz, P. D. Adams, D. Saxena, R. J. Meagher and A. K. Singh, Lab Chip, 2014, 14, 4533
    DOI: 10.1039/C4LC00823E

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