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Issue 21, 2014
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An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria

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Abstract

In this paper, for the first time, an on-chip optofluidic imaging system is innovated to measure the biophysical signatures of single waterborne bacteria, including both their refractive indices and morphologies (size and shape), based on immersion refractometry. The key features of the proposed optofluidic imaging platform include (1) multiple sites for single-bacterium trapping, which enable parallel measurements to achieve higher throughput, and (2) a chaotic micromixer, which enables efficient refractive index variation of the surrounding medium. In the experiments, the distinctive refractive index of Echerichia coli, Shigella flexneri and Vibrio cholera are measured with a high precision of 5 × 10−3 RIU. The developed optofluidic imaging system has high potential not only for building up a database of biophysical signatures of waterborne bacteria, but also for developing single-bacterium detection in treated water that is in real-time, label-free and low cost.

Graphical abstract: An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria

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Publication details

The article was received on 05 Jul 2014, accepted on 14 Aug 2014 and first published on 14 Aug 2014


Article type: Paper
DOI: 10.1039/C4LC00783B
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2014,14, 4237-4243
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    An optofluidic imaging system to measure the biophysical signature of single waterborne bacteria

    P. Y. Liu, L. K. Chin, W. Ser, T. C. Ayi, P. H. Yap, T. Bourouina and Y. Leprince-Wang, Lab Chip, 2014, 14, 4237
    DOI: 10.1039/C4LC00783B

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