Issue 19, 2014
From the journal:

Lab on a Chip

Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique†

Samad Ahadian,a   Shukuyo Yamada,b   Javier Ramón-Azcón,*acd   Kosuke Ino,b   Hitoshi Shiku,b   Ali Khademhosseiniaefghi  and  Tomokazu Matsueab  

* Corresponding authors

a WPI-Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan
E-mail: ramonazconjavier@gmail.com
Fax: +81 22 217 6140
Tel: +81 22 217 6140

b Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan

c Department of Chemical and Biomolecular Nanotechnology, Advanced Chemical Research Institute of Catalonia (IQAC-CSIC), Jordi Girona 18-26, Barcelona 08034, Spain

d Nanobiotechnology for Diagnostics (Nb4D) Group, IQAC-CSIC, Jordi Girona 18-26, Barcelona 08034, Spain

e Department of Medicine, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA

f Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

g Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA

h Department of Physics, King Abdulaziz University, Jeddah 21569, Saudi Arabia

i Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea

Abstract

In this manuscript, we demonstrate the rapid formation of three-dimensional (3D) embryonic stem cell (ESC) aggregates with controllable sizes and shapes in hydrogels using dielectrophoresis (DEP). The ESCs encapsulated within a methacrylated gelatin (GelMA) prepolymer were introduced into a DEP device and, upon applying an electric field and crosslinking of the GelMA hydrogel, formed 3D ESC aggregates. Embryoid bodies (EBs) fabricated using this method showed high cellular viability and pluripotency. The proposed technique enables production of EBs on a large scale and in a high-throughput manner for potential cell therapy and tissue regeneration applications.

Graphical abstract: Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique

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Article information

DOI
https://doi.org/10.1039/C4LC00479E
Article type
Communication
Submitted
23 Apr 2014
Accepted
23 Jul 2014
First published
23 Jul 2014

Lab Chip, 2014,14, 3690-3694

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Rapid and high-throughput formation of 3D embryoid bodies in hydrogels using the dielectrophoresis technique

S. Ahadian, S. Yamada, J. Ramón-Azcón, K. Ino, H. Shiku, A. Khademhosseini and T. Matsue, Lab Chip, 2014, 14, 3690 DOI: 10.1039/C4LC00479E

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