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Issue 15, 2014
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Improved electrochemical detection of a transthyretin synthetic peptide in the nanomolar range with a two-electrode system integrated in a glass/PDMS microchip

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Abstract

An alternative to a three-electrode set-up for electrochemical detection and analysis in microfluidic chips is described here. The design of the electrochemical sensor consists of the surface of the glass substrate covered with a PDMS block which bears the microfluidic channels. A band microelectrode which acts as a working electrode surrounded by a large counter electrode is obtained at the micrometric level to propose a simple and efficient sensing area for on-a-chip analysis. The counter-electrode with a surface area about 22-fold greater than the working-microelectrode can also be considered as a pseudo reference since its current density is low and thus limits the potential variations around the rest potential. To this purpose, the [Fe(III)(CN)6]3−/[Fe(II)(CN)6]4− redox couple was used in order to set a reference potential at 0 V since both electrodes were platinum. The electrochemical microchip performance was characterized using differential pulse voltammetric (DPV) detection and quantification of the optically multi-labelled transthyretin synthetic peptide mimicking a tryptic fragment of interest for the diagnosis of familial transthyretin amyloidosis (ATTR). The limit of detection of the peptide by the working microelectrode was 25 nM, a value 100-fold lower than the one reported with conventional capillary electrophoresis coupled with laser-induced fluorescence under the same analytical conditions.

Graphical abstract: Improved electrochemical detection of a transthyretin synthetic peptide in the nanomolar range with a two-electrode system integrated in a glass/PDMS microchip

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Publication details

The article was received on 25 Feb 2014, accepted on 16 May 2014 and first published on 16 May 2014


Article type: Paper
DOI: 10.1039/C4LC00240G
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Citation: Lab Chip, 2014,14, 2800-2805
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    Improved electrochemical detection of a transthyretin synthetic peptide in the nanomolar range with a two-electrode system integrated in a glass/PDMS microchip

    M. Faure, A. Pallandre, S. Chebil, I. Le Potier, M. Taverna, B. Tribollet, C. Deslouis, A. Haghiri-Gosnet and J. Gamby, Lab Chip, 2014, 14, 2800
    DOI: 10.1039/C4LC00240G

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