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Issue 6, 2014
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The intersection of flow cytometry with microfluidics and microfabrication

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Abstract

A modern flow cytometer can analyze and sort particles on a one by one basis at rates of 50 000 particles per second. Flow cytometers can also measure as many as 17 channels of fluorescence, several angles of scattered light, and other non-optical parameters such as particle impedance. More specialized flow cytometers can provide even greater analysis power, such as single molecule detection, imaging, and full spectral collection, at reduced rates. These capabilities have made flow cytometers an invaluable tool for numerous applications including cellular immunophenotyping, CD4+ T-cell counting, multiplex microsphere analysis, high-throughput screening, and rare cell analysis and sorting. Many bio-analytical techniques have been influenced by the advent of microfluidics as a component in analytical tools and flow cytometry is no exception. Here we detail the functions and uses of a modern flow cytometer, review the recent and historical contributions of microfluidics and microfabricated devices to field of flow cytometry, examine current application areas, and suggest opportunities for the synergistic application of microfabrication approaches to modern flow cytometry.

Graphical abstract: The intersection of flow cytometry with microfluidics and microfabrication

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Publication details

The article was received on 09 Oct 2013, accepted on 14 Jan 2014 and first published on 15 Jan 2014


Article type: Tutorial Review
DOI: 10.1039/C3LC51152A
Author version available: Download Author version (PDF)
Citation: Lab Chip, 2014,14, 1044-1059
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    The intersection of flow cytometry with microfluidics and microfabrication

    M. E. Piyasena and S. W. Graves, Lab Chip, 2014, 14, 1044
    DOI: 10.1039/C3LC51152A

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