Jump to main content
Jump to site search

Issue 3, 2014
Previous Article Next Article

Ligand density elicits a phenotypic switch in human neutrophils

Author affiliations

Abstract

Neutrophils are mediators of innate immunity and motility is critical to their function. We used microcontact printing to investigate the relationship between density of adhesive ligands and the dynamics of neutrophil motility. We show that neutrophils adopt a well-spread morphology without a uropod on moderate densities of adhesion ligand. As density is increased, the morphology switches to a classic amoeboid shape. In addition to the morphological differences, the dynamics of motility were quantitatively distinct. Well-spread cells without uropods glide slowly with high persistence, while amoeboid cells made frequent directional changes migrating quickly with low persistence. Using an antibody panel against various integrin chains, we show that adhesion and motility on fibronectin are mediated by MAC-1 (αMβ2). The phenotypic switch could be generalized to other surface ligands, such as bovine serum albumin, to which the promiscuous MAC-1 also binds. These results suggest that neutrophils are capable of displaying multiple modes of motility as dictated by their adhesive environment.

Graphical abstract: Ligand density elicits a phenotypic switch in human neutrophils

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 28 Oct 2013, accepted on 05 Jan 2014 and first published on 10 Jan 2014


Article type: Paper
DOI: 10.1039/C3IB40225H
Citation: Integr. Biol., 2014,6, 348-356
  •   Request permissions

    Ligand density elicits a phenotypic switch in human neutrophils

    S. J. Henry, J. C. Crocker and D. A. Hammer, Integr. Biol., 2014, 6, 348
    DOI: 10.1039/C3IB40225H

Search articles by author