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Issue 10, 2014
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Non-invasive mapping of interstitial fluid pressure in microscale tissues

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Abstract

This study describes a non-invasive method for mapping interstitial fluid pressure within hydrogel-based microscale tissues. The method is based on embedding (or forming) a tissue within a silicone (PDMS) microfluidic device, and measuring the extremely slight displacement (<1 μm) of the PDMS optically when the device is pressurized under static and flow conditions. The displacement field under uniform pressure provides a map of the local device stiffness, which can then be used to obtain the non-uniform pressure field under flow conditions. We have validated this method numerically and applied it towards determining the hydraulic properties of tumor cell aggregates, blind-ended epithelial tubes, and perfused endothelial tubes that were all cultured within micropatterned collagen gels. The method provides an accessible tool for generating high-resolution maps of interstitial fluid pressure for studies in mechanobiology.

Graphical abstract: Non-invasive mapping of interstitial fluid pressure in microscale tissues

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Publication details

The article was received on 15 Jul 2014, accepted on 27 Aug 2014 and first published on 28 Aug 2014


Article type: Technical Innovation
DOI: 10.1039/C4IB00164H
Citation: Integr. Biol., 2014,6, 979-987
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    Non-invasive mapping of interstitial fluid pressure in microscale tissues

    O. Ozsun, R. L. Thompson, K. L. Ekinci and J. Tien, Integr. Biol., 2014, 6, 979
    DOI: 10.1039/C4IB00164H

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