Jump to main content
Jump to site search

Issue 7, 2014
Previous Article Next Article

Activation of macrophages stimulated by the bengkoang fiber extract through toll-like receptor 4

Author affiliations

Abstract

Bengkoang (Pachyrhizus erosus (L.) Urban) is an edible root tuber containing fairly large amounts of carbohydrates and crude fibers. Our previous studies showed that the bengkoang fiber extract (BFE) stimulates activation of macrophages, leading to induction of phagocytotic activity and cytokine production. In the present study we investigated the mechanism underlying activation of murine macrophages by BFE. BFE increased production of TNF-α, IL-6, and nitric oxide by J774.1 cells. In addition BFE also facilitated the gene expression levels of inducible nitric oxide synthase. We examined the effect of a TLR4 inhibitor on cytokine production to investigate the membrane receptor of macrophage activation by BFE. Treatment of J774.1 cells with the TLR4 inhibitor significantly inhibited production of IL-6 and TNF-α, suggesting that TLR4 is the target membrane receptor for BFE. The main signal molecules located downstream of TLR4 such as JNK, p38, ERK, and NF-κB were activated by BFE treatment. The immunostimulatory effect of BFE was cancelled by the pectinase treatment, suggesting that the active ingredient in BFE is pectin-like molecules. Overall results suggested that BFE activates J774.1 cells via the MAPK and NF-κB signaling pathways.

Graphical abstract: Activation of macrophages stimulated by the bengkoang fiber extract through toll-like receptor 4

Back to tab navigation

Publication details

The article was received on 28 Aug 2013, accepted on 17 Mar 2014 and first published on 28 Apr 2014


Article type: Paper
DOI: 10.1039/C3FO60360A
Citation: Food Funct., 2014,5, 1403-1408
  •   Request permissions

    Activation of macrophages stimulated by the bengkoang fiber extract through toll-like receptor 4

    I. D. Kumalasari, K. Nishi, A. B. N. Putra and T. Sugahara, Food Funct., 2014, 5, 1403
    DOI: 10.1039/C3FO60360A

Search articles by author

Spotlight

Advertisements