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Issue 18, 2014
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Protein film photoelectrochemistry of the water oxidation enzyme photosystem II

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Abstract

Photosynthesis is responsible for the sunlight-powered conversion of carbon dioxide and water into chemical energy in the form of carbohydrates and the release of O2 as a by-product. Although many proteins are involved in photosynthesis, the fascinating machinery of Photosystem II (PSII) is at the heart of this process. This tutorial review describes an emerging technique named protein film photoelectrochemistry (PF-PEC), which allows for the light-dependent activity of PSII adsorbed onto an electrode surface to be studied. The technique is straightforward to use, does not require highly specialised and/or expensive equipment, is highly selective for the active fractions of the adsorbed enzyme, and requires a small amount of enzyme sample. The use of PF-PEC to study PSII can yield insights into its activity, stability, quantum yields, redox behaviour, and interfacial electron transfer pathways. It can also be used in PSII inhibition studies and chemical screening, which may prove useful in the development of biosensors. PSII PF-PEC cells also serve as proof-of-principle solar water oxidation systems; here, a comparison is made against PSII-inspired synthetic photocatalysts and materials for artificial photosynthesis.

Graphical abstract: Protein film photoelectrochemistry of the water oxidation enzyme photosystem II

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Publication details

The article was received on 17 Jan 2014 and first published on 26 Mar 2014


Article type: Tutorial Review
DOI: 10.1039/C4CS00031E
Citation: Chem. Soc. Rev., 2014,43, 6485-6497
  • Open access: Creative Commons BY license
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    Protein film photoelectrochemistry of the water oxidation enzyme photosystem II

    M. Kato, J. Z. Zhang, N. Paul and E. Reisner, Chem. Soc. Rev., 2014, 43, 6485
    DOI: 10.1039/C4CS00031E

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      [Original citation] - Published by The Royal Society of Chemistry.

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