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Issue 31, 2014
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Accessing the third dimension in localization-based super-resolution microscopy

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Abstract

Only a few years after its inception, localization-based super-resolution microscopy has become widely employed in biological studies. Yet, it is primarily used in two-dimensional imaging and accessing the organization of cellular structures at the nanoscale in three dimensions (3D) still poses important challenges. Here, we review optical and computational techniques that enable the 3D localization of individual emitters and the reconstruction of 3D super-resolution images. These techniques are grouped into three main categories: PSF engineering, multiple plane imaging and interferometric approaches. We provide an overview of their technical implementation as well as commentary on their applicability. Finally, we discuss future trends in 3D localization-based super-resolution microscopy.

Graphical abstract: Accessing the third dimension in localization-based super-resolution microscopy

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Publication details

The article was received on 30 Mar 2014, accepted on 16 May 2014, published on 05 Jun 2014 and first published online on 05 Jun 2014


Article type: Perspective
DOI: 10.1039/C4CP01380H
Citation: Phys. Chem. Chem. Phys., 2014,16, 16340-16348
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    Accessing the third dimension in localization-based super-resolution microscopy

    B. Hajj, M. El Beheiry, I. Izeddin, X. Darzacq and M. Dahan, Phys. Chem. Chem. Phys., 2014, 16, 16340
    DOI: 10.1039/C4CP01380H

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