Issue 46, 2014
From the journal:

Chemical Communications

A fluorescent light-up probe as an inhibitor of intracellular β-tryptase†

Qi Wang,‡a   Xiuyin Shi,‡b   Xiaoxia Zhu,a   Martin Ehlers,c   Junchen Wu*d  and  Carsten Schmuck*c  

* Corresponding authors

a College of Public Health, Nantong University, 9 Seyuan Road, Nantong, China

b Medical Laboratory Center, Affiliated Hospital of Nantong University, Nantong, China

c Institute for Organic Chemistry, University of Duisburg-Essen, Universitatsstrasse 7, Germany
E-mail: carsten.schmuck@uni-due.de
Fax: +49 201 183 4259
Tel: +49 201 183 3097

d Key Lab for Advanced Materials and Institute of Fine Chemicals, East China University of Science and Technology, China
E-mail: jcwu@ecust.edu.cn
Fax: +86 21 6425 2258
Tel: +86 21 6425 3674

Abstract

A pyrene-functionalized peptidic inhibitor 1 binds to and inhibits β-tryptase in a non-competitive and reversible manner even in cells. Upon protein binding a fluorescence increase of the two pyrene fluorophores is observed which allows using 1 as a fluorescent light-up probe for this enzyme.

Graphical abstract: A fluorescent light-up probe as an inhibitor of intracellular β-tryptase

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Article information

DOI
https://doi.org/10.1039/C4CC02208D
Article type
Communication
Submitted
01 Apr 2014
Accepted
14 Apr 2014
First published
28 Apr 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2014,50, 6120-6122

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A fluorescent light-up probe as an inhibitor of intracellular β-tryptase

Q. Wang, X. Shi, X. Zhu, M. Ehlers, J. Wu and C. Schmuck, Chem. Commun., 2014, 50, 6120 DOI: 10.1039/C4CC02208D

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