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Issue 28, 2014
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Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

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Abstract

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.

Graphical abstract: Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

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Publication details

The article was received on 21 Jan 2014, accepted on 17 Feb 2014 and first published on 28 Feb 2014


Article type: Communication
DOI: 10.1039/C4CC00540F
Citation: Chem. Commun., 2014,50, 3747-3749
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    Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

    K. Hsieh, P. L. Mage, A. T. Csordas, M. Eisenstein and H. Tom Soh, Chem. Commun., 2014, 50, 3747
    DOI: 10.1039/C4CC00540F

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