Sensitive detection of polynucleotide kinase using rolling circle amplification-induced chemiluminescence

Abstract

We develop a new method for the sensitive detection of polynucleotide kinase (PNK) using rolling circle amplification-induced chemiluminescence. This method exhibits high sensitivity with a detection limit of 2.20 × 10⁻⁴ U mL⁻¹, which is superior to most reported approaches. Moreover, this method can be used to screen both the inhibitors and the activators of PNK, and can be further applied for real sample analysis.