Immunoassays on nanopatterned chips through TIRS detection based on reconstructing the three dimensional position provided a nanoscale accuracy of the lateral resolution by using the z-stage controller in the spatial range up to 10 nm. This method offers highly accurate and sensitive quantification with the zeptomolar (∼10−21 M) detection of proteins.
S. Lee, H. Yu and S. H. Kang, Chem. Commun., 2015, 51, 945 DOI: 10.1039/C4CC07665F
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