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Issue 85, 2014
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Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography

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Abstract

Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity.

Graphical abstract: Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography

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Publication details

The article was received on 24 Jul 2014, accepted on 05 Sep 2014 and first published on 05 Sep 2014


Article type: Communication
DOI: 10.1039/C4CC05771F
Citation: Chem. Commun., 2014,50, 12903-12906
  • Open access: Creative Commons BY license
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    Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography

    J. Lietard, N. Kretschy, M. Sack, A. S. Wahba, M. M. Somoza and M. J. Damha, Chem. Commun., 2014, 50, 12903
    DOI: 10.1039/C4CC05771F

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