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Issue 80, 2014
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A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases

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Abstract

We have developed a rapid and high-throughput assay based on rolling circle amplification, to distinguish individual strand cleavage of DNA duplexes by restriction endonucleases. As an illustration, we analyzed nicking activity of Nb.BbvCI and uneven cleavage of LNA modified DNA by EcoRI. This assay has potential for analyzing protein–DNA interactions.

Graphical abstract: A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases

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Publication details

The article was received on 12 Jul 2014, accepted on 13 Aug 2014 and first published on 13 Aug 2014


Article type: Communication
DOI: 10.1039/C4CC05314A
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2014,50, 11930-11933
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    A RCA-based assay for analyzing individual strand break in DNA heteroduplex cleavage by restriction endonucleases

    G. Zhao, T. Hu, J. Li, H. Wei, H. Shang and Y. Guan, Chem. Commun., 2014, 50, 11930
    DOI: 10.1039/C4CC05314A

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