Native chemical ubiquitination using a genetically incorporated azidonorleucine

Renliang Yang; Xiaobao Bi; Fupeng Li; Yuan Cao; Chuan-Fa Liu

doi:10.1039/C4CC03721A

Native chemical ubiquitination using a genetically incorporated azidonorleucine

Renliang Yang,^a Xiaobao Bi,^a Fupeng Li,^a Yuan Cao^a and Chuan-Fa Liu*^a

* Corresponding authors

^a Division of Structural Biology and Biochemistry, School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551
E-mail: cfliu@ntu.edu.sg
Fax: +65 67953021

Abstract

A robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1–75)-thioester and the target protein. To demonstrate our methodology, a model protein, K48-linked diubiquitin, was synthesized with an overall yield of 35%.

Supplementary files

Publication details

The article was received on 15 May 2014, accepted on 04 Jun 2014 and first published on 04 Jun 2014

Article type: Communication

DOI: 10.1039/C4CC03721A

Author version available: Download Author version (PDF)

Citation: Chem. Commun., 2014,50, 7971-7974

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Native chemical ubiquitination using a genetically incorporated azidonorleucine

R. Yang, X. Bi, F. Li, Y. Cao and C. Liu, Chem. Commun., 2014, 50, 7971
DOI: 10.1039/C4CC03721A
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Chemical Communications

Native chemical ubiquitination using a genetically incorporated azidonorleucine

Abstract

Supplementary files

Publication details

Native chemical ubiquitination using a genetically incorporated azidonorleucine

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