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Issue 65, 2014
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A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

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Abstract

A general combined purification and immobilization method to facilitate biocatalytic process development is presented. The support material, EziG™, is based on controlled porosity glass (CPG) or polymer-coated versions thereof (HybCPG) and binds protein affinity tags. Biocatalytic reactions in aqueous and organic media with seven enzymes of biocatalytic interest are shown.

Graphical abstract: A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

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Publication details

The article was received on 08 Apr 2014, accepted on 20 Jun 2014 and first published on 03 Jul 2014


Article type: Communication
DOI: 10.1039/C4CC02605E
Citation: Chem. Commun., 2014,50, 9134-9137
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    A general protein purification and immobilization method on controlled porosity glass: biocatalytic applications

    K. Engelmark Cassimjee, M. Kadow, Y. Wikmark, M. Svedendahl Humble, M. L. Rothstein, D. M. Rothstein and J.-E. Bäckvall, Chem. Commun., 2014, 50, 9134
    DOI: 10.1039/C4CC02605E

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