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Issue 43, 2014
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Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions

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Abstract

A simple and efficient protocol for the preparative-scale synthesis of various lengths of oligo-N-acetyllactosamine (oligo-LacNAc) and its multi-sialylated extensions is described. The strategy utilizes one thermophilic bacterial thymidylyltransferase (RmlA) coupled with corresponding sugar-1-phosphate kinases to generate two uridine diphosphate sugars, UDP-galactose and UDP-N-acetylglucosamine. By incorporating glycosyltransferases, oligo-LacNAcs and their sialylated analogs were synthesized.

Graphical abstract: Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions

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Publication details

The article was received on 17 Feb 2014, accepted on 11 Apr 2014 and first published on 11 Apr 2014


Article type: Communication
DOI: 10.1039/C4CC01227E
Author version available: Download Author version (PDF)
Citation: Chem. Commun., 2014,50, 5786-5789
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    Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions

    W. Chien, C. Liang, C. Yu, C. Lin, S. Li, I. Primadona, Y. Chen, K. K. T. Mong and C. Lin, Chem. Commun., 2014, 50, 5786
    DOI: 10.1039/C4CC01227E

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